CGSC Strain#: 6451
Strain Designation: K603      Source of Strain: N. Zinder
Sex: F+ Episome/Plasmid: F1-10(Tn10)
   Plasmid Markers/Mutations: z--1::Tn10
   Plasmid Comment: F1::Tn10, the integration of Tn10 in F1 to give F1-10 does not interfere with transfer functions.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, xylA5, mtl-1, thiE1
Strain Comments:

• The integration of Tn 10 in F1 to give F1-10 does not interfere with transfer functions.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• = tetracycline resistant

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