CGSC Strain#: 12386
Strain Designation: STL13719      Source of Strain: S.T. Lovett
Sex: F- Chromosomal Markers: lacZ4807(Am), mhpC281::Tn10, λ^-, rph-1
Strain Comments:

• lacZ4807(Am)-- is a 2 basepair substitution (GAA to TAG) in the Glu-461 codon resulting in an amber stop codon.
• mhpC281::Tn10-- cotransduces 80% with lac.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• lacZ4807(Am) was formerly called lacZ(T1384G)
• mhpC281::Tn10 was formerly called zah281::Tn10
• = tetracycline resistant
• Am = amber(UAG) mutation

References:

• Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.