CGSC Strain#: 12393
Strain Designation: STL13817     
Source of Strain: S.T. Lovett
Sex: F-
Chromosomal Markers: lacZ4815,
mhpC281::Tn10,
λ-,
rph-1Strain Comments: - lacZ4815-- is a 2 basepair insertion of a CG residue pair causing a frameshift mutation at base 1520 of the lacZ gene..
- mhpC281::Tn10-- cotransduces 80% with lac.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- lacZ4815 was formerly called lacZ(-2CG1520)
- mhpC281::Tn10 was formerly called zah281::Tn10
- = tetracycline resistant
References: - Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.