CGSC Strain#: 6493
Strain Designation: DP50      Source of Strain: D. Pereira
Other Designations: χ1953
Other Designations: chi1953
Sex: F- Chromosomal Markers: fhuA53, dapD8, lacY1 or Δ(cod-lacI)6, glnX44(AS), Δ(gal-uvrB)47, λ^-, gyrA29(NalR), ΔthyA57, hsdS3
Strain Comments:

• dapD8-- Strains with this mutation require 100ug/ml diaminopimelic acid even when grown on LB
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• ΔthyA57-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

References:

• Leder, P, D Tiemeier, L Enquist 1977. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system. Science 196(4286):175-7.