CGSC Strain#: 6406
Strain Designation: RW231      Source of Strain: R. Wolf
Sex: F- Chromosomal Markers: lacZ4503(Am), λ^-, trpA9605(Am), kdgR50, Δ(eda-zwf)209, Δ(sbcB-rfb)210, recA1, rpsL125(strR), trpR55
Strain Comments:

• trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Wolf, RE 1980. Integration of specialized transducing bacteriophage lambda cI857 St68 h80 dgnd his by an unusual pathway promotes formation of deletions and generates a new translocatable element. J. Bacteriol. 142(2):588-602.