CGSC Strain#: 3561
Strain Designation: T4^RT6^R      Source of Strain: E.A. Adelberg
Other Designations: AB3561
Sex: F- Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), galK2(Oc), Rac-0, rfbC1, rpsL20(strR), xylA5, mtl-1, thiE1, tfr-5, pyr-27
Strain Comments:

• This strain may also carry the mutation lct-1. For discussion of membrane defect in parent strain AB1621 see Schnaitman 1974 J. Bacteriol. 118: 454.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Adelberg, E.A., M. Mandel, G.C.C. Chen 1965. Optimal conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochem.Biophys.Res.Commun. 18:788-795