CGSC Strain#: 12549
Strain Designation: CM742     
Source of Strain: Sex: F-
Chromosomal Markers: ara-19,
fhuA1,
lacY1 or lacZ4,
tsx-33,
glnX44(AS)
,
galK2(Oc)
,
λ-,
Rac-0,
hisG4(Oc)
,
rfbC1,
rpsL8 or rplL9(L?)
,
mtl-1,
dnaA46(ts)
,
metE46,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Marsh, NJ, DE Duggan 1972. Ordering of mutant sites in the isoleucine-valine genes of Escherichia coli by use of merogenotes derived from F 14 : a new procedure for fine-structure mapping. J. Bacteriol. 109(2):730-40.
- Wegrzyn, G, A Wegrzyn, I Konieczny, K Bielawski, G Konopa, M Obuchowski, DR Helinski, K Taylor 1995. Involvement of the host initiator function dnaA in the replication of coliphage lambda. Genetics 139(4):1469-81.