CGSC Strain#: 4001
Strain Designation: TS-40     
Source of Strain: A. Boyd
Other Designations: AB4001
Sex: F-
Chromosomal Markers: galK2(Oc)
,
trp-5,
hisG4(Oc)
,
mtl-1,
argE3(Oc)
,
thiE1,
tsm-11(DNA(ts)
)Strain Comments: - galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- tsm-11(DNA(ts))-- is a DNA temperature-sensitive mutation. It shows high residual incorporation of 3H-thymine at 42 degrees C.
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- DNA(ts) = DNA temperature-sensitive
- Oc = ochre(UAA) mutation
References: - Boyd, D, R Nixon, S Gillespie, D Gillespie 1968. Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation. J. Bacteriol. 95(3):1040-50.