CGSC Strain#: 4363
Strain Designation: KL190     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: galK35,
λ-,
pyrD34,
trpC45,
his-68,
thyA25,
thyA33,
rpsL118(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1Strain Comments: - Some of the allele numbers are given incorrectly in the reference. This strain is said to be suppressor-free.
- galK35-- is Brenner's gal-U99 from Hfr 3000 U99 and has been called galK99
- thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- thyA33-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Godson, GN 1973. Isolation and partial characterization of temperature-sensitive mutants in ten loci that affect cell membrane synthesis in Escherichia coli: isolation and genetic sorting. J. Bacteriol. 113(2):813-24.