CGSC Strain#: 4603
Strain Designation: DS2      Source of Strain: D.A. Steege
Sex: F- Chromosomal Markers: phoA4(Am), trp-37, his-45, recA1, rpsL114(strR)
Strain Comments:

• This strain was made by D. steege in Wilhelm's laboratory to replace HW-1A2, which had been lost. It is Val^R. (Reference to HW-1A2.)
• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Hoffman, E.P., R. Wilhelm 1970. Genetic mapping and dominance of the amber suppressor, Su1 (supD) in Escherichia coli K-12. J.Bacteriol. 103:32-36