CGSC Strain#: 5283
Strain Designation: PLS73      Source of Strain: G.N. Godson
Sex: F- Chromosomal Markers: ΔphoA8, galK35, λ^-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1, pls-73
Strain Comments:

• Due to misinformation sent out by CGSC, some of the allele numbers are given incorrectly in the reference.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• strR = streptomycin resist.
• T2R = T2 Resistant

References:

• Godson, GN 1973. Isolation and partial characterization of temperature-sensitive mutants in ten loci that affect cell membrane synthesis in Escherichia coli: isolation and genetic sorting. J. Bacteriol. 113(2):813-24.