CGSC Strain#: 5726
Strain Designation: Lin 292     
Source of Strain: E.C.C. Lin
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510,
mgr-2Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- mgr-2-- mgr is a symbol provisionally assigned to the mutation which confers resistance to methylglyoxal on this strain.
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Freedberg, W.B., W.S. Kistler, E.C.C. Lin 1971. Lethal synthesis of methylglyoxal by Escherichia coli during unregulated glycerol metabolism. J.Bacteriol. 108:137-144