CGSC Strain#: 5737
Strain Designation: RFS857      Source of Strain: R. Schleif
Sex: F- Chromosomal Markers: thr-1, araB857(polar), leuB6(Am), Δ(codB-lacI)3, tsx-274, λ^-, gyrA111(NalR), recA111, relA1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• NalR = Nalidix.acidResist.
• polar = has polar effects downstream

References:

• Schleif, R 1972. Fine-structure deletion map of the Escherichia coli L-arabinose operon. Proc. Natl. Acad. Sci. U.S.A. 69(11):3479-84.