CGSC Strain#: 5767
Strain Designation: MI226      Source of Strain: M. Iaccarino
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: thr-10, car-94, λ^-, relA1, spoT1, ilvG603(Act), ilvG605(Am), thiE1
Strain Comments:

• lyophil opened 9/03
• This strain is Ilv^- due to the ilvGAm mutation
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ilvG603(Act)-- is an insertion mutation which activates the ilvG gene.
• ilvG605(Am)-- is an amber mutation.
• thiE1 was formerly called thi-1
• Act = activates the Gene
• Am = amber(UAG) mutation

References:

• De Felice, M., J. Guardiola, W. Schreil, M. Levinthal, M. Iaccarino 1977. Metabolic interlock between the acetolactate synthase isoenzymes and lysine biosynthesis in Escherichia coli K-12. Mol.Gen.Genet. 156:9-16