CGSC Strain#: 5790
Strain Designation: RF-7     
Source of Strain: R.H. Fillingame
Sex: F-
Chromosomal Markers: glnX44(AS)
,
galK2(Oc)
,
λ-,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
tfr-3,
dcc-1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- dcc-1-- may be an uncB or uncE mutation.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Fillingame, R.H. 1975. Identification of the dicyclohexylcarbodiimide-reactive protein component of the adenosine 5'-triph9osphate transducing system of Escherichia coli. J.Bacteriol. 124:870-883