CGSC Strain#: 5922
Strain Designation: JW164     
Source of Strain: J.A. Wechsler
Sex: F-
Chromosomal Markers: lacZ53(Am)
,
λ-,
e14-,
thyA36,
rpsL151(strR)
,
polA1(Am)
,
rha-5,
malB45,
deoC2Strain Comments: - lacZ53(Am)-- is the Paris lac-Y14 amber.
- e14--- : Loss of the e14 element makes the strain McrA-.
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMSS,UVS, viable at all temps. Grindley MGG143
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: