CGSC Strain#: 5929
Strain Designation: K-99     
Source of Strain: R. Calendar
Sex: F-
Chromosomal Markers: fhuA2::IS2,
lacY1 or lacZ4,
tsx-7,
glnX44(AS)
,
λ-,
hisG1,
gltB32,
rpsL8 or rplL9(L?)
or rpsL17,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1,
ΔargH1,
thiE1Strain Comments: - The gltB allele was found in parent strain AB1450 (Berberich 1972 Biochem.Biophys.Res.Commun. 47:1498).
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- = strep-resistant
- ::IS2 = Insertion of IS2
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Calendar, R., B. Lindquist, G. Sironi, A.J. Clark 1970. Characterization of REP^- mutants and their interaction with P2 phage. Virology 40:72-83