CGSC Strain#: 6316
Strain Designation: CAG1     
Source of Strain: R.R. Burgess
Sex: F-
Chromosomal Markers: lacY1 or lacZ4,
tsx-64,
glnX44(AS)
,
gal-6,
hisG1,
metC69,
rpoD800(ts)
,
argG6,
rpsL8 or rpsL104 or rpsL17,
malT1(λ
R)
Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- metC69-- was called metC58 by A.L. Taylor.
- rpoD800(ts)-- is a temperature-sensitive mutation.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- = strep-resistant
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- ts = Temperature Sensitive
References: - Liebke, H., C.A. Gross, W. Walter, R.R. Burgess 1980. A new mutation rpoD800, affecting the sigma subunit of E. coli RNA polymerase is allelic to two other sigma mutants. Mol.Gen.Genet. 177:277-282