CGSC Strain#: 5818
Strain Designation: ASH114     
Source of Strain: I.B. Holland
Sex: Hfr
Point of Origin#: 130,129
Map Position: 13.00
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ-,
thyA117,
rpsL228(strR)
,
metB1,
creD114Strain Comments: - thyA117-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- strR = streptomycin resist.
References: - Buxton, R.S., I.B. Holland 1973. Genetic studies of tolerance to colicin E2 in Escherichia coli K12. I. Re-location and dominance relationships of cet mutations. Mol.Gen.Genet. 127:69-88