CGSC Strain#: 14233
Strain Designation: WTZ19001      Source of Strain: J.E. Wertz
Other Designations: JK378 - rsgA
Sex: F- Chromosomal Markers: ara-600, Δ(gpt-lac)5, λ^-, zdi-925::Tn10, infC362, relA1, spoT1, thiE1, ΔrsgA787::kan
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• zdi-925::Tn10-- cotransduces ca. 65% with pps and 55% with pfkB. Found by PCR (Nichols et al. 1998) to be an insertion at nucleotide 1,776,386 (38.291 min.)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ΔrsgA787::kan-- The primers used to make this knockout were GACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAATTCCGGGGATCCGTCGACC and GTATCATAGATGTTTTGCCCATCAGGGGCGACCAGGAGTCTGTACGATTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• thiE1 was formerly called thi-1
• = tetracycline resistant
• = kanamycin resistant

References:

• Sussman, J.K., E.L. Simons, R.W. Simons 1996. Escherichia coli translation initiation factor 3 discriminates the initiation codon in vivo. Mol.Microbiol. 21:347-360