CGSC Strain#: 13798
Strain Designation: pLC19-24      Source of Strain:
Sex: F+ Episome/Plasmid: pLC19-24
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, nrdA , nrdB , glpQ , glpT
   Plasmid Comment: Hybridizes with Kohara clones 376-379. Carries nrdA at 50.5, as well as nrdB, glpQ, and glpT at 50.6.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
• Nishimura, Y., Y. Takeda, A. Nishimura, H. Suzuki, M. Inouye, Y. Hirota 1977. Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coli. Plasmid 1:67-77
• Weiner, JH, E Lohmeier, A Schryvers 1978. Cloning and expression of the glycerol-3-phosphate transport genes of Escherichia coli. Can. J. Biochem. 56(6):611-7.
• Yamada, M., Y. Takeda, K. Okamoto, Y. Hirota 1982. Physical map of the nrdA-nrdB-ftsB-glpT region of the chromosomal DNA of Escherichia coli. Gene 18:309-318
• Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151