CGSC Strain#: 6431
Strain Designation: EK25     
Source of Strain: R.A. Kelln
Sex: F-
Chromosomal Markers: leuB6(Am)
,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-6,
hisG1,
recA56,
srlD300::Tn10,
argG6,
argR64,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1,
pyrB43Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- recA56-- Amino acid substitution Arg 60 Cys
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- srlD300::Tn10 was formerly called srlC300::Tn10
- xyl-7 was formerly called xylA7
- = strep-resistant
- = tetracycline resistant
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: