CGSC Strain#: 6431
Strain Designation: EK25      Source of Strain: R.A. Kelln
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, hisG1, recA56, srlD300::Tn10, argG6, argR64, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1, pyrB43
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• recA56-- Amino acid substitution Arg 60 Cys
• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• srlD300::Tn10 was formerly called srlC300::Tn10
• xyl-7 was formerly called xylA7
• = strep-resistant
• = tetracycline resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References: