CGSC Strain#: 5106
Strain Designation: W1485-E      Source of Strain: E.M. Lederberg
Other Designations: Paris Lyophil #11436
Sex: F+ Chromosomal Markers: glnV42(AS), λ^-, rpoS396(Am), rph-1
Strain Comments:

• Old "computer card" says "This is an early version of W1485 and therefore should not contain supE42."
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• glnV42(AS) was formerly called supE42
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Lederberg, J., E.M. Lederberg 1953. Genetic studies of lysogenicity in Escherichia coli. Genetics 38:51-64
• Taylor, A.L., C. Yanofsky 1964. Transformation of bacterial markers and transfer of phage markers with DNA isolated form LAM-Phi80 hybrid phage carrying the tryptophan genes of E. coli. Biochem.Biophys.Res.Commun. 17:798-804
• Vallari, D.S., C.O. Rock 1985. Isolation and characterization of Escherichia coli pantothenate permease (panF) mutants. J.Bacteriol. 164:136-142
• Jensen, K.F. 1993. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J.Bacteriol. 175:3401-3407