CGSC Strain#: 4933
Strain Designation: his Dab472      Source of Strain:
Sex: Hfr    Point of Origin#:    Map Position: Chromosomal Markers: Δ(gpt-proA)62, lacY1, lacZ4, glnX44(AS), galK2(Oc), hisD472, purF1, rpsL8, xylA5, thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• purF1-- is a very stable mutation and may be an intragenic deletion.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Garrick-Silversmith, L., P.E. Hartman 1970. Histidine-requiring mutants of Escherichia coli K12. Genetics 66:231-244