CGSC Strain#: 4398
Strain Designation: KL340      Source of Strain:
Sex: F' Episome/Plasmid: F42-17
   Plasmid Markers/Mutations: lacI22, lacZ608(Am), lac
Chromosomal Markers: Δ(gpt-lac)5, φ80dlacI⁺, Δ(att80-tonB)886, rpsE2129(SpcR), xyl-7, mtlA2
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• φ80dlacI⁺-- The ?80dlacI⁺ was constructed by integrating the ?80dlac⁺ prophage and then selecting for tonB mutants that also deleted the lacZYA genes leaving the lacZ promoter.
• Δ(att80-tonB)886-- This deletion may extend beyond tonB
• xyl-7 was formerly called xylA7
• SpcR = spectinomycin resistant
• Am = amber(UAG) mutation

References:

• Lloyd, RG, B Low 1976. Some genetic consequences of changes in the level of recA gene function in Escherichia coli K-12. Genetics 84(4):675-95.