CGSC Strain#: 6709
Strain Designation: DJK432     
Source of Strain: D. Kolodrubetz
Sex: F-
Chromosomal Markers: thr-42,
araD,
araC,
Δ(codB-lacI)3,
λ-,
araF432,
his-182,
gyrA270(NalR)
,
relA1,
araE79,
spoT1,
thiE1Strain Comments: - araC and araD allele numbers not assigned because derivation information is incomplete
- Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- thiE1 was formerly called thi-1
- NalR = Nalidix.acidResist.
References: - Kolodrubetz, D. 1980. Thesis, Brandeis University 0:26-29