CGSC Strain#: 7173
Strain Designation: PRC436      Source of Strain:
Sex: F+ Chromosomal Markers: fadR201, tyrT58(AS), adhE36, adhEp-81, mel-1
Strain Comments:

• adhE36-- Little or no ACDH activity, but near wild-type levels of ADH. Confers resistance to chloroacetaldehyde (8 ug/ml) in an adhEp-81 background
• adhEp-81-- is an operator or promoter mutation. It results in 60-fold overproduction of ADH on succinate plus acetate medium and allows growth on EtOH as sole C source.
• adhEp-81 was formerly called adhC81
• tyrT58(AS) was formerly called supF58
• AS = amber(UAG) suppressor

References:

• Cunningham, P.R., D.P. Clark 1986. The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol.Gen.Genet. 205:487-493
• Cunningham, P.R., D.P. Clark 1986. The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol.Gen.Genet. 205:487-493