CGSC Strain#: 7442
Strain Designation: CAG12153     
Source of Strain: C.A. Gross
Sex: F-
Chromosomal Markers: λ-,
zhc-6::Tn10,
rph-1Strain Comments: - zhc-6::Tn10-- cotransduces 15% with fabE, 17% with argG, 2% with panF. PCR analysis (Nichols et al.1998) places the insertion within the orf b3219 (yhcF), at nucleotide 3,364,914 (75.52 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = tetracycline resistant
References: - Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.