CGSC Strain#: 7459
Strain Designation: CAG18558      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, rph-1, zid-3162::Tn10kan
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• zid-3162::Tn10kan-- is derived from zid-501::Tn10 which cotransduces ca. 90% with dnaA and gyrB and was shown by PCR (Nichols et al.1998) to be at bp 3,387,001(83.484 min.)
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.