CGSC Strain#: 7344
Strain Designation: CAG12021      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: zbd-3104::Tn10, λ^-, rph-1
Strain Comments:

• Transduction results subsequent to the PCR analysis did not confirm the presence of gatY::Tn (feedback '04) and that marker has been removed from this strain.
• zbd-3104::Tn10-- Although PCR analysis placed the Tn10 insertion in CAG12021 within gatY, ca. 46.9', subsequent transductions did not find Tn cotransducing with markers in the 45-49' region(feedback '04)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• zbd-3104::Tn10 was formerly called zbd-3105
• = tetracycline resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.