CGSC Strain#: 7448
Strain Designation: CAG12133      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, acrF3171::Tn10kan, rph-1
Strain Comments:

• acrF3171::Tn10kan-- Derived from acrF3083(zhe-3083)::Tn10, shown by PCR analysis (Nichols et al. 1998 J.Bacteriol. 180:6408) to be within the acrF gene. at nucleotide 3,412,834 (minute 74.565)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• acrF3171::Tn10kan was formerly called zje-3171
• acrF3171::Tn10kan was formerly called zhe-3171
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of