CGSC Strain#: 7279
Strain Designation: TK614     
Source of Strain: R. Woodgate
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
glnX44(AS)
,
galK2(Oc)
,
λ-,
umuD77,
Rac-0,
hisG4(Oc)
,
rpsL31(strR)
,
xylA5,
mtl-1,
ilv-325,
thiE1,
uvrA6Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Koch, W.H., D.G. Ennis, A.S. Levine, R. Woodgate 1992. Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage. Mol.Gen.Genet. 233:443-448