CGSC Strain#: 7512
Strain Designation: CAG12200      Source of Strain: C.A. Gross
Other Designations: CSH132
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: zed-3128::Tn10kan, relA1, spoT1, thiE1
Strain Comments:

• zed-3128::Tn10kan is an insertion in the orf yedZ (b1972), at nucleotide 2,038,762 (min. 43.946)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.