CGSC Strain#: 6300
Strain Designation: MG1655      Source of Strain: M.S. Guyer
Sex: F- Chromosomal Markers: λ^-, rph-1
Strain Comments:

• This culture was grown from lyophil Oct. 2003 and it tested Fnr⁺. The previous working culture was mixed fnr^-/fnr⁺.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• Guyer, M.S., R.E. Reed, T. Steitz, K.B. Low 1981. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spr.Harb.Symp.Quant.Biol. 45:135-140
• Jensen, K.F. 1993. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J.Bacteriol. 175:3401-3407
• Blattner, FR, G Plunkett, CA Bloch, NT Perna, V Burland, M Riley, J Collado-Vides, JD Glasner, CK Rode, GF Mayhew, J Gregor, NW Davis, HA Kirkpatrick, MA Goeden, DJ Rose, B Mau, Y Shao 1997. The complete genome sequence of Escherichia coli K-12. Science 277(5331):1453-74.