CGSC Strain#: 4260
Strain Designation: KL729      Source of Strain: M.C. Cappello
Other Designations: KLF12
Sex: F' Episome/Plasmid: F112
   Plasmid Markers/Mutations: malB28(λ^R)
   Plasmid Comment: may carry mal-28(LamR)
Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ^-, hisG1, recA1, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• 1.This strain should be maintained on medium containing arg, his, leu and lacking met. It grows slowly. Watch out for fast-growing clones that have lost all or part of F112. F112 may carry
• 2. mal-28(LamR)
• See JC1553, Chart 9, Bachmann's Derivations and Genotypes paper.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• LamR = Lambda-resistant

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.