CGSC Strain#: 6092
Strain Designation: DL16     
Source of Strain: D.M. LeMaster
Sex: F-
Chromosomal Markers: leu-64,
lacZ53(Am)
,
λ-,
recA1,
rpsL151(strR)
,
ilvE720::Tn5,
trxA1(Am)
,
metE70,
rha-5,
malB45,
deoC2Strain Comments: - lacZ53(Am)-- is the Paris lac-Y14 amber.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- trxA1(Am)-- Gln98 to Amber codon. Growth of WT phage f1 in this strain is TS on all suppressors, whereas T7 grows at 42C.
- = kanamycin resistant
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Mark, D.F., J.W. Chang, C.F. Richardson 1977. Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12. Mol.Gen.Genet. 155:145-152