CGSC Strain#: 5024
Strain Designation: W1485      Source of Strain: J. Lederberg
Sex: F+ Chromosomal Markers: λ^-, rpoS396(Am), rph-1
Strain Comments:

• rpoS396(Am) was reported to be present in W1485 by Visick and Clarke, J.Bact. 179:4158. It is not cited as the CGSC 5024 culture, so we are not certain of its presence here.
• This strain does not carry a suppressor mutation and it does not have Tn1000 inserted into the chromosome, as do some derivatives of this strain which have been called simply ``W1485."
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Am = amber(UAG) mutation

References:

• Lederberg, J., E.M. Lederberg 1953. Genetic studies of lysogenicity in Escherichia coli. Genetics 38:51-64
• Guyer, M.S., R.E. Reed, T. Steitz, K.B. Low 1981. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spr.Harb.Symp.Quant.Biol. 45:135-140
• Jensen, K.F. 1993. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J.Bacteriol. 175:3401-3407