CGSC Strain#: 5041
Strain Designation: NH4920      Source of Strain: J. Hall
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, hisG4(Oc), recA44(ts), rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, deoB16
Strain Comments:

• The strain does not grow well at high temperature. Use 42 degrees as the restrictive and 32 degrees as the permissive temp.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA44(ts)-- is a temperature-sensitive mutation.
• recA44(ts)-- G to A at Nuc. 977 (Val 247 Met)
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Hall, JD, P Howard-Flanders 1975. Temperature-sensitive recA mutant of Escherichia coli K-12: deoxyribonucleic acid metabolism after ultraviolet irradiation. J. Bacteriol. 121(3):892-900.