CGSC Strain#: 5067
Strain Designation: N1625      Source of Strain: S. Gottesman
Sex: F- Chromosomal Markers: λ^-, relA1, rpsL150(strR), spoT1, bglR6, lop-8
Strain Comments:

• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• strR = streptomycin resist.

References:

• Gottesman, MM, ML Hicks, M Gellert 1973. Genetics and function of DNA ligase in Escherichia coli. J. Mol. Biol. 77(4):531-47.