CGSC Strain#: 5099
Strain Designation: AN59      Source of Strain: F. Gibson
Sex: Hfr    Point of Origin#: 13   Map Position: 84.98   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-1, leuB6(Am), lacZ4, glnX44(AS), P1+, rpsL8, fre-352, thiE1
Strain Comments:

• This strain probably carries phage P1.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Cox, E.C., F. Gibson, J. Pittard 1968. Mutant strains of E.coli K-12 unable to form ubiquinone. J.Bacteriol. 95:1591-0
• Cox, GB, IG Young, LM McCann, F Gibson 1969. Biosynthesis of ubiquinone in Escherichia coli K-12: location of genes affecting the metabolism of 3-octaprenyl-4-hydroxybenzoic acid and 2-octaprenylphenol. J. Bacteriol. 99(2):450-8.