CGSC Strain#: 856
Strain Designation: JC158     
Source of Strain: A.J. Clark
Other Designations: AB856
Other Designations: AT2475
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacI22,
λ-,
e14-,
relA1,
serA6,
spoT1,
thiE1Strain Comments: - e14--- : Loss of the e14 element makes the strain McrA-.
- serA6-- grows well on 100ug/ml Ser and poorly on 20ug/ml, serA6 is tight. (In JC158).
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- lacI22 was formerly called laci3-
- lacI22 was formerly called lacI3
- thiE1 was formerly called thi-1
References: - Clark, A.J. 1963. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics 48:105-120