CGSC Strain#: 5477
Strain Designation: Ac64      Source of Strain: U. Henning
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: thr-26, aceE64, λ^-, e14-, trp-66, relA1, spoT1, thiE1
Strain Comments:

• aceE64 is an amber mutation. Also note sex is Hfr?.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Henning, U. 1966. Cold Spr.Harb.Symp.Quant.Biol. 31:227-0
• Dietrich, J, U Henning 1970. Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K 12. Identification of the inducing metabolite. Eur. J. Biochem. 14(2):258-69.