CGSC Strain#: 5474
Strain Designation: LEA145     
Source of Strain: E. Yagil
Sex: F-
Chromosomal Markers: secA206(aziR)
,
fhuA23,
lacZ73,
lacI22,
proC34,
tsx-67,
glnX44(AS)
,
λ-,
trpE38,
rpsL109(strR)
,
xylA5,
mtl-1,
thiE1,
P1RStrain Comments: - This is a spontaneous pur+ derivative of strain LE145.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- P1R-- This is a spontaneous pur+ derivative of strain LE145.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- lacI22 was formerly called lacI3
- lacI22 was formerly called laci3-
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- aziR = azide resistance
- strR = streptomycin resist.
References: - Bracha, M, E Yagil 1973. A ne type of alkaline phosphatase-negative mutants in Escherichia coli K12. Mol. Gen. Genet. 122(1):53-60.