CGSC Strain#: 5081
Strain Designation: Hfr 3000 U482      Source of Strain: F. Jacob
Other Designations: HfrC U482
Other Designations: HfrH U482
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, asd-1, spoT1, thiE1
Strain Comments:

• Requires diaminopimelic acid for growth in rich medium.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Hatfield, D., M. Hofnung, M. Schwartz 1969. Genetic analysis of the maltose A region in Escherichia coli. J.Bacteriol. 98:559-567