CGSC Strain#: 5112
Strain Designation: JP5061      Source of Strain: R.R.B. Russell
Sex: F- Chromosomal Markers: araC14, leuB6(Am), secA206(aziR), fhuA23, tsx-67, purE42, glnX44(AS), nagB2, galE45(GalS), λ^-, rpsL109(strR), xylA5, mtl-1, rpoB301, thiE1
Strain Comments:

• May carry malA1 and suppressor for Mal near his (from grandparent ``AB1515,rif^R").
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galE45(GalS)-- is from strain PL5 of Buttin.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• GalS = UDPGal accum.toxic if GalKRP+
• strR = streptomycin resist.

References:

• White, R.J. 1968. Control of amino sugar metabolism in Escherichia coli and isolation of mutants unable to degrade amino sugars. Biochem.J. 106:847-858
• Holmes, RP, RR Russell 1972. Mutations affecting amino sugar metabolism in Escherichia coli K-12. J. Bacteriol. 111(1):290-1.