CGSC Strain#: 7565
Strain Designation: DS121     
Source of Strain: S. Rahuel-Clermont
Sex: F-
Chromosomal Markers: λ-,
Δepd-11::Ery,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - These gap/epd-deleted strains should be grown on minimal supplemented with casamino acids, Trp, Met, Thi, succinate and glycerol plus antibiotic. They will not grow on LB or glucose media.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- Δepd-11::Ery was formerly called gapB11::Erm
References: - Seta, F.D., S. Boschi-Muller, M.L. Vignais, G. Branlant 1997. Characterization of Escherichia coli strains with gapA and gapB genes deleted. J.Bacteriol. 179:5218-5221