CGSC Strain#: 5198
Strain Designation: NP315     
Source of Strain: Bock & Neidhardt
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
fbaA2(ts)
,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- fbaA2(ts) was formerly called fda-2
- Const = constitutive
- T2R = T2 Resistant
- ts = Temperature Sensitive
References: - Bock, A., F.C. Neidhardt 1966. Isolation of a mutant of Escherichia coli with a temperature-sensitive fructose-1,6-diphosphate aldolase activity. J.Bacteriol. 92:464-469