CGSC Strain#: 5574
Strain Designation: E112     
Source of Strain: H.C. Wu
Sex: F-
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-33,
nagB3,
glnS1(ts)
,
galE28(GalS)
,
λ-,
rpsL31(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - This strain should be grown with only 20ug/ml N-acetylglucosamine, as higher concentrations are inhibitory. This strain has an additional, unknown auxotrophic requirement.
- glnS1(ts)-- is a temperature-sensitive mutation. Growth stops immediately at 42 degrees C. This allele reverts and the strain should be cloned and tested before use.
- Δ(gpt-proA)62 was formerly called proA2
- thiE1 was formerly called thi-1
- GalS = UDPGal accum.toxic if GalKRP+
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Wu, HC, TC Wu 1971. Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase. J. Bacteriol. 105(2):455-66.