CGSC Strain#: 5381
Strain Designation: LS853     
Source of Strain: L. Soll
Sex: F-
Chromosomal Markers: λ-,
trpA9605(Am)
,
his-85(Am)
,
IN(rrnD-rrnE)1,
ΔcyaA2,
trpR55Strain Comments: - trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
- his-85(Am)-- is an amber mutation. It is suppressed by supD32.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- ΔcyaA2-- is a 75-bp in-frame deletion (Shah and Peterkofsky, 1991). Earlier feedback had sometimes reported that this allele `reverts' or is `unstable'.
- trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
- ΔcyaA2 was formerly called cyaA854
- his-85(Am) was formerly called his-29
- Am = amber(UAG) mutation
References: - Brickman, E., L. Soll, J.R. Beckwith 1973. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J.Bacteriol. 116:582-587
- Brickman, E., L. Soll, J.R. Beckwith 1973. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J.Bacteriol. 116:582-587