CGSC Strain#: 5393
Strain Designation: E111     
Source of Strain: H.C. Wu
Sex: F-
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-33,
galE28(GalS)
,
λ-,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
glmS1,
argE3(Oc)
,
thiE1Strain Comments: - There have been reports that the glmS marker in this strain is leaky or has reverted
- This strain must be grown on 200ug/ml N-acetylglucosamine, preferably in PPBE medium (see paper cited below).
- Δ(gpt-proA)62 was formerly called proA2
- thiE1 was formerly called thi-1
- GalS = UDPGal accum.toxic if GalKRP+
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Wu, HC, TC Wu 1971. Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase. J. Bacteriol. 105(2):455-66.